gfp rab7 q67l (Addgene inc)
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Addgene inc
gfp rab7 q67l
Gfp Rab7 Q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab7+q67l/ZP39+En3+(Plasmid+%2316903)/pmc12583604-30-0-3
Average 93 stars, based on 4 article reviews
Gfp Rab7 Q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rab7+q67l/ZP39+En3+(Plasmid+%2316903)/pmc12583604-30-0-3
Average 93 stars, based on 4 article reviews
gfp rab7 q67l - by Bioz Stars,
2026-09
93/100 stars
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Recombinant:Article Title: Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility Article Snippet: Recombinant DNA reagent , GFP-Rab5 Q79L , Addgene , 28046 , . .. Recombinant DNA reagent , Plasmid Preparation:Article Title: Human VPS13A is associated with multiple organelles and influences mitochondrial morphology and lipid droplet motility Article Snippet: BFP-Sec61B (Addgene plasmid #49154) and mCherry-Sec61B (Addgene plasmid #49155) were kind gifts from Gia Voeltz ( ). .. GFP-Rab5 Q79L (Addgene plasmid #28046) and Article Title: SKIP ‐ HOPS recruits TBC 1D15 for a Rab7‐to‐Arl8b identity switch to control late endosome transport Article Snippet: All cell lines were maintained at 37 degrees, 5% CO 2 , and routinely (negatively) tested for mycoplasma contamination (Lonza, LT07‐318). .. GFP‐Rab7 and Myc‐Rab7 have been described before (Jordens et al , ), and Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 532 cDNAs were purchased from Origene, followed by Gateway cloning into the pHAGE-C-533 terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA or pHAGE-GFP vector 534 (CCZ1). .. GFP-RAB7 WT , other:Article Title: BLTP3A is associated with membranes of the late endocytic pathway and is an effector of CASM Article Snippet: Article Title: Endosomal trafficking participates in lipid droplet catabolism to maintain lipid homeostasis Article Snippet: Mutagenesis:Article Title: SKIP ‐ HOPS recruits TBC 1D15 for a Rab7‐to‐Arl8b identity switch to control late endosome transport Article Snippet: All cell lines were maintained at 37 degrees, 5% CO 2 , and routinely (negatively) tested for mycoplasma contamination (Lonza, LT07‐318). .. GFP‐Rab7 and Myc‐Rab7 have been described before (Jordens et al , ), and Dominant Negative Mutation:Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 cDNAs were purchased from Origene and subjected to Gateway cloning into the pHAGE-C-terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA, or pHAGE-GFP vector (CCZ1). .. GFP-RAB7 WT , Article Title: Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux Article Snippet: C18orf8 and CCZ1 532 cDNAs were purchased from Origene, followed by Gateway cloning into the pHAGE-C-533 terminal-FLAG-HA vector (C18orf8), pHAGE-N-terminal-FLAG-HA or pHAGE-GFP vector 534 (CCZ1). .. GFP-RAB7 WT , |
![C18orf8/RMC1 is required for endolysosomal size control and autophagic flux. (A) HeLa cells stably expressing RMC1-FLAG-HA were grown on glass coverslips, fixed, and immunostained as indicated. (B) HeLa cells were transfected with control or RMC1 siRNA pools; at 72 h posttransfection, cells were harvested for immunoblot analysis with the indicated antibodies. (C) Cells treated with siRNAs as for panel B were grown on glass coverslips, fixed, and immunostained with the late endosomal marker anti-CD63; maximum-intensity projections of z-stacks are shown, and scale bars represent 20 μm. (D) Cells treated with siRNAs as for panel B were fixed and analyzed by TEM. (E) HeLa cells were treated with individual or combined siRNAs as indicated for 72 h, followed by immunoblot analysis to evaluate depletion of RMC1 and accumulation of p62 protein. (F) Quantification of p62 levels shown in panel E for two biological replicate experiments. Error bars represent standard deviation, and significance was determined by one-way ANOVA with post hoc testing (Dunnett's multiple-comparison test). *, P < 0.05; **, P < 0.01. (G) HeLa cells were grown on glass coverslips, fixed, and immunostained with anti-CD63 and LC3B; representative maximum-intensity projections are shown, and scale bars represent 20 μm. (H) Quantification of basal LC3B punctum number in cells stained as for panel G. Data represent two pooled biological experiments, and error bars represent standard deviation. (I and J) HeLa cells treated with control or RMC1 siRNAs (as for panel E) were treated with Cell Light <t>RFP-GFP-LC3B</t> for 16 h, followed by incubation in nutrient-replete DMEM (I) or starvation medium (HBSS) (J) for 1 h. Autophagic flux was monitored by confocal microscopy; single z-sections are shown for each channel, and scale bars represent 20 μm. (K and L) Quantification of average percentage of RFP-GFP-positive and RFP-only puncta per cell as shown in panels I and J. Data represent two pooled biological experiments, error bars represent standard deviation of the mean, and significance was determined by one-way ANOVA with post hoc testing (Dunnett's multiple-comparison test). ****, P < 0.0001. (M) Left panel, HeLa cells were grown on glass coverslips in nutrient-replete medium, fixed, and stained with endogenous antibodies against <t>RAB7</t> and CD63. Individual representative z-sections for each channel are shown, and scale bars represent 20 μm. Right panel, intensity profiles of a 20-μm line segment drawn across the z-section shown for each channel. Overlap of 488 (RAB7) and 561 (CD63) fluorescence intensity profiles (arbitrary units [AU]) indicates colocalization of RAB7 with the late endosomal marker CD63.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_0722/pmc05730722/pmc05730722__zmb0011816710007.jpg)
